Multiple imputation models for SARS-CoV-2 viral load

Abstract Number:

2785 

Submission Type:

Contributed Abstract 

Contributed Abstract Type:

Paper 

Participants:

Gail Potter (1), Allyson Mateja (2), Eric Chu (3), Daniel Rubin (4), David Smith (5), Victor De Gruttola (6)

Institutions:

(1) National Institute of Health / NIAID, Rockville, MD, (2) Clinical Monitoring Research Program Directorate, Frederick National Laboratory for Cancer Research, Frederick MD, (3) Clinical Monitoring Research Program Directorate, Frederick National Laboratory for Cancer Research, Frederick, MD, (4) FDA, Washington, DC, (5) University of California, San Diego, San Diego, CA, (6) Harvard University, Cambridge MA

Co-Author(s):

Allyson Mateja  
Clinical Monitoring Research Program Directorate, Frederick National Laboratory for Cancer Research
Eric Chu  
Clinical Monitoring Research Program Directorate, Frederick National Laboratory for Cancer Research
Daniel Rubin  
FDA
David Smith  
University of California, San Diego
Victor DeGruttola  
Harvard University

First Author:

Gail Potter  
National Institute of Health / NIAID

Presenting Author:

Gail Potter  
National Institute of Health / NIAID

Abstract Text:

SARS-CoV-2 viral load is frequently used as an endpoint in Phase 2 COVID-19 treatment trials. Meta-analyses of multiple trials have missingness by design due to different sampling schedules, missingness not by design, and left-truncation at the lower limit of quantification (LLOQ). We compare three viral load imputation models. The first treats infection status as a latent variable. For uninfected people, viral load is represented as a point mass at zero; for infected people, viral load is modeled as lognormally distributed with left-truncation at LLOQ. Hence, the allotment of probability mass <LLOQ to infected and uninfected people is driven by the untestable assumption of log normality of viral load values <LLOQ for infected people. To avoid the need for this assumption, a second approach directly models "<LLOQ" as a point mass and values above LLOQ as lognormally distributed. Temporal correlation is modeled by inclusion of individual-level random intercepts. A third approach involves individual linear interpolation plus random noise estimated from the empirical covariance structure. We compare the models in simulation and apply them to COVID-19 treatment trials in outpatients.

Keywords:

multiple imputation|viral load|longitudinal data|latent variable|mixture model|interpolation

Sponsors:

Biometrics Section

Tracks:

Missing Data

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