Tuesday, Aug 6: 10:30 AM - 12:20 PM
2673
Contributed Posters
Oregon Convention Center
To understand heart aging at the single-cell level, we employed single-cell dual omics (scRNA and scATAC) in non-myocytes (non-CMs) from young (3m), middle-aged (12m), and elderly (24m) mice. Non-CMs, vital in heart development, physiology, and pathology, are understudied compared to cardiomyocytes. Our analysis revealed aging response heterogeneity among non-CM cell types. Immune cells, notably macrophages and neutrophils, showed significant aging alterations, while endothelial cells displayed moderate changes. We identified distinct aging signatures within the cell type, including differential gene expression and transcription factor activity, along with motif variation. Sub-cluster analysis revealed intra-cell type heterogeneity, characterized by diverse aging patterns. The senescence-associated secretory phenotype (SASP) emerged as a key aging-related phenotype. Moreover, aging significantly influenced cell-cell communication, especially impacting a fibroblast sub-cluster, Fib.Erbb4. This study elucidates the complex cellular and molecular landscape of cardiac aging in non-CMs, highlighting their importance in heart aging and offering potential therapeutic avenues.
Cardiac Aging
Single-cell Dual omics
Non-myocytes
Aging Heterogeneity
Senescence-Associated Secretory Phenotype (SASP)
Fibroblast Sub-Cluster
Main Sponsor
Section on Statistics in Genomics and Genetics