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51 Decoding Aging in the Heart via Single Cell Dual Omics of Non-Cardiomyocytes

Li Wang Co-Author
Medical Research Institute, Wuhan University
 
Li Qian Co-Author
The University of North Carolina
 
Jiandong Liu Co-Author
The University of North Carolina at Chapel Hill
 
Yiran Song First Author
 
Yiran Song Presenting Author
 
Tuesday, Aug 6: 10:30 AM - 12:20 PM
2673 
Contributed Posters 
Oregon Convention Center 
To understand heart aging at the single-cell level, we employed single-cell dual omics (scRNA and scATAC) in non-myocytes (non-CMs) from young (3m), middle-aged (12m), and elderly (24m) mice. Non-CMs, vital in heart development, physiology, and pathology, are understudied compared to cardiomyocytes. Our analysis revealed aging response heterogeneity among non-CM cell types. Immune cells, notably macrophages and neutrophils, showed significant aging alterations, while endothelial cells displayed moderate changes. We identified distinct aging signatures within the cell type, including differential gene expression and transcription factor activity, along with motif variation. Sub-cluster analysis revealed intra-cell type heterogeneity, characterized by diverse aging patterns. The senescence-associated secretory phenotype (SASP) emerged as a key aging-related phenotype. Moreover, aging significantly influenced cell-cell communication, especially impacting a fibroblast sub-cluster, Fib.Erbb4. This study elucidates the complex cellular and molecular landscape of cardiac aging in non-CMs, highlighting their importance in heart aging and offering potential therapeutic avenues.

Keywords

Cardiac Aging

Single-cell Dual omics

Non-myocytes

Aging Heterogeneity

Senescence-Associated Secretory Phenotype (SASP)

Fibroblast Sub-Cluster 

Abstracts


Main Sponsor

Section on Statistics in Genomics and Genetics